Test Directory

FLT3

Containers - Adult

Red Cap Tube EDTA KE 9ml	Volume Range 2 x 9ml peripheral blood, bone marrow specimen or FFPE tissue. Total volume required is 10-15mL. Sample tube can be any size as long as it is EDTA anti-coagulated Additive per Container EDTA

Laboratory Site

WGH

Crewe Road South
Edinburgh
EH4 2XU

Telephone: 0131 537 1000

Transport arrangements

Specimens should ideally arrive within 24 hours of collection (maximum of 48 hours) and should be sent to the following address:

Western General Hospital

Haematology/ Biochemistry Combined Reception

Immunophenotyping Laboratory

Crewe Road

Edinburgh

EH4 2XU

Sample storage arrangements

Samples should be stored at room temperature. See specimen requirements.

How to request

Please refer to our detailed requesting instructions. The HMDS request form can be located here.

HMDS request form PDF_v5.pdf

Testing may be performed by reflex from immunophenotyping.

Availability

Monday-Friday 9am-5pm

Anticipated turnaround

Results should be expected within 3 working days. See results.

Static information/disclaimer

This test is accredited to ISO 15189:2012

General additional information

FLT3 (both ITD and point mutation D835) gene mutation analysis performed for all patients diagnosed with AML based on immunophenotyping results via a reflex testing strategy.

FLT3 mutation is not a reliable marker for MRD monitoring within AML

FLT3 is the most commonly mutated gene in AML and detected in one third of AML cases. Internal tandem duplications (ITDs) in the juxtamembrane domain of FLT3 are seen in 25% of AML cases, while others show mutations in the tyrosine kinase domain of FLT3 gene.

Where FLT3 ITD mutation detected, the mutant allele ratio of the ITD mutation can be calculated. The presence of a high level of FLT3 ITD mutation in the absence of a NPM1 mutation is associated with an adverse prognosis in AML.

NPM1 mutation detection is also available for AML patients at diagnosis.

For clinical advice on appropriate investigations and advice for the interpretation of test results, please contact us.