Test Directory


Containers - Adult

Red Cap Tube EDTA KE 9ml
Volume Range

2 x 9ml peripheral blood, bone marrow specimen or FFPE tissue.

Total volume required is 10-15mL.  Sample tube can be any size as long as it is EDTA anti-coagulated.

Additive per Container


Laboratory Site

Crewe Road South
Telephone: 0131 537 1000

Transport arrangements

Specimens should ideally arrive within 24 hours of collection (maximum of 48 hours) and should be sent to the following address:

Western General Hospital

Haematology/Biochemistry Combined Reception

Immunophenotyping Laboratory

Crewe Road


EH4 2XU​


See also Specimen transportation.

Sample storage arrangements

Samples should be stored at room temperature. See specimen requirements.​

How to request

Please refer to our detailed requesting ​instructions.  The HMDS request form can be located here.​



​Monday-Friday 9am-5pm

Anticipated turnaround

Results should be expected within 3 working days.  See results.

Static information/disclaimer

This test is accredited to ISO 15189:2012

General additional information

NPM1 gene mutation analysis performed for all patients diagnosed with AML based on immunophenotyping results via a reflex testing strategy.

Mutation in the NPM1 gene is observed in approximately 30% of AML cases and is frequently associated with a normal karyotype. The presence of an NPM1 mutation in the absence of a FLT3 internal tandem duplication  is associated with a more favourable prognosis in AML.

FLT3 (ITD and D835) mutation detection is also available for AML patients at diagnosis. 

For patients carrying an NPM1 mutation for whom molecular monitoring will be required, identification of the mutation type will be determined by next generation sequencing or Sanger sequencing.

Molecular monitoring for NPM1 positive AML is available for types A, B & D.

For NPM1 MRD monitoring, refer to the separate entry in the test directory, found here

For clinical advice on appropriate investigations and advice for the interpretation of test results, please contact us.