Test Directory

Immunoglobulin HV Gene Sequencing

Containers - Adult

Red Cap Tube EDTA KE 9ml
Volume Range

5-10 ml peripheral blood or bone marrow specimen

Additive per Container


Laboratory Site

Crewe Road South
Telephone: 0131 537 1000

Transport arrangements

Specimens should ideally arrive within 24 hours of collection (maximum of 48 hours) and should be sent to the following address:

Western General Hospital

Haematology/Biochemistry Combined Reception

Immunophenotyping Laboratory

Crewe Road


EH4 2XU​


See also Specimen transportation.

Sample storage arrangements

Samples should be stored at room temperature. See specimen requirements.​


How to request

Please refer to our detailed requestinginstructions.  The HMDS request form can be located here.​


Monday - Friday 9am-5pm

Anticipated turnaround

Results should be expected within 15 working days.  See results.​​

Static information/disclaimer

This test is accredited to ISO15189:2012.

General additional information

This assay will ascertain the mutation status of the IGHV immunoglobulin locus in patients with chronic lymphocytic leukaemia. IGHV gene mutation status is determined by the percentage homology to the germline sequence.

Homology of ≥ 98% to germline is regarded as unmutated (U-CLL), whereas a homology of < 97% is regarded as mutated (M-CLL). Homology 97-97.9% is considered borderline and therefore should be interpreted with caution.

Patients with U-CLL have a less favourable outcome compared to patients with M-CLL.

Assessment the TP53 gene by sanger sequencing can also be performed.

For clinical advice on appropriate investigations and advice for the interpretation of test results, please contact us​.