Test Directory

POLE mutation analysis

Containers - Adult

path_block_slide.jpg
FFPE block / H&E slide / Pathology report
Volume Range

Referral FFPE specimens should be sent directly to Molecular Pathology at RIE

If DNA has already been extracted, this may be sent to Molecular Pathology/HMDS at the WGH site.

Laboratory Site

RIE
51 Little France Crescent
Old Dalkeith Road
Edinburgh
EH16 4SA
Telephone: 0131 536 1000
WGH
Crewe Road South
Edinburgh
EH4 2XU
Telephone: 0131 537 1000

Transport arrangements

Referral specimens should be sent directly to Molecular Pathology at the above address (see transport recommendations). For patients with pathology specimens held within NHS Lothian there is no need to arrange transport of specimens.

If DNA has already been extracted, this may be sent to Molecular Pathology/HMDS at the WGH site.

How to request

Testing for NHS Lothian patients can be requested by email to molecular.pathoogy@nhslothian.scot.nhs.uk.

Referral requests must be accompanied by a completed request form.

Please also refer to our detailed requesting instructions.

Availability

Monday - Friday. 09:00 – 17:00

Anticipated turnaround

An integrated Molecular Pathology report should be available within 10 working days. See results.

Static information/disclaimer

This test has been accredited to ISO15189. However, part of the procedure now makes use of an automated pipetting system which has been separately validated, but is not currently accredited to ISO 15189.

DNA extraction is performed at RIE and subsequent Sanger sequencing is performed at WGH.

Please note: alternative methodologies may be used. Full details will be included in all reports.

General additional information

Assessment of POLE (exons 9 to 14) is available for endometrial carcinoma and, along with assessment of TP53 and MMR/MSI, provides information regarding prognosis and therapy.

For clinical advice on appropriate investigations, please contact our Molecular Pathology team.