Test Directory

MPL exon 10

Containers - Adult

RedCapTubeEDTAKE2.7ml.jpg
K2 EDTA KE Blood (Pink Top)

Reference ranges

​Not detected.

Laboratory Site

RIE
51 Little France Crescent
Old Dalkeith Road
Edinburgh
EH16 4SA
Telephone: 0131 536 1000

Transport arrangements

Specimens should be kept at room temperature and sent directly to the laboratory as soon as possible by first class post.

Sample storage arrangements

Specimens must NOT be frozen. If a delay in sending a specimen is unavoidable it must be refrigerated overnight.

How to request

Request form, or can be added onto a previous JAK2 V617F sample via the MPN Mailbox: Molecular.MPN@nhslothian.scot.nhs.uk

Availability

Test not available during restricted service

Anticipated turnaround

28 days

What happens if the result is positive or abnormal

Genetic results will not be telephoned. They can be emailed if secure addresses are verified.

Static information/disclaimer

​This test is accredited to ISO 15189:2012​​​

General additional information

Variants in MPL exon 10 are tested for in patients with clinical details of “Essential Thrombocythaemia' or 'Myelofribrosis” and have previously tested 'Not Detected' for both JAK2 V617F and Calreticulin indel Mutations. The MPL exon 10 is screened using an HRM method, followed by direct sequencing for the confirmation and classification of any potential mutations detected. This HRM method is sensitive to approximately 5-10%.

See the relevant BCSH guidelines for further information:

https://b-s-h.org.uk/guidelines/guidelines/gpp-use-of-genetic-tests-to-diagnose-and-manage-patients-with-myeloproliferative-and-myeloproliferativemyelodysplastic-neoplasms-and-related-disorders/

Citrated anticoagulated venous blood or 150µl DNA may be sent instead of an EDTA specimen