Test Directory
MPL exon 10
Containers - Adult
K2 EDTA KE Blood (Pink Top)
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Reference ranges
Not detected.
Laboratory Site
Old Dalkeith Road
Edinburgh
EH16 4SA
Transport arrangements
Specimens should be kept at room temperature and sent directly to the laboratory as soon as possible by first class post.
Sample storage arrangements
Specimens must NOT be frozen. If a delay in sending a specimen is unavoidable it must be refrigerated overnight.
How to request
Request form, or can be added onto a previous JAK2 V617F sample via the MPN Mailbox: Molecular.MPN@nhslothian.scot.nhs.uk
Availability
Test not available during restricted service
Anticipated turnaround
28 days
What happens if the result is positive or abnormal
Genetic results will not be telephoned. They can be emailed if secure addresses are verified.
Static information/disclaimer
This test is accredited to ISO 15189:2022
General additional information
Variants in MPL exon 10 are tested for in patients with clinical details of “Essential Thrombocythaemia' or 'Myelofribrosis” and have previously tested 'Not Detected' for both JAK2 V617F and Calreticulin indel Mutations. The MPL exon 10 is screened using an HRM method, followed by direct sequencing for the confirmation and classification of any potential mutations detected. This HRM method is sensitive to approximately 5-10%.
See the relevant BCSH guidelines for further information:
Citrated anticoagulated venous blood or 150µl DNA may be sent instead of an EDTA specimen