Test Directory
Calreticulin (CALR exon 9)
Containers - Adult
K2 EDTA KE Blood (Pink Top)
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Reference ranges
Not Detected.
Laboratory Site
Old Dalkeith Road
Edinburgh
EH16 4SA
Transport arrangements
Specimens should be kept at room temperature and sent directly to the laboratory as soon as possible by first class post.
Sample storage arrangements
Specimens must NOT be frozen. If a delay in sending a specimen is unavoidable it must be refrigerated overnight.
How to request
Request form, or can be added onto a previous JAK2 V617F sample via the MPN Mailbox: Molecular.MPN@nhslothian.scot.nhs.uk
Availability
Test not available during restricted service.
Anticipated turnaround
28 days
What happens if the result is positive or abnormal
Genetic results will not be telephoned. They can be emailed if secure addresses are verified.
Static information/disclaimer
This test is accredited to ISO 15189:2012
General additional information
Mutations in Calreticulin Exon 9 are tested for in patients with Essential Thrombocythaemia or Myelofibrosis and have previously tested 'Not Detected' for JAK2 V617F.
Frameshift indels within exon 9 of CALR are detected by fragment analysis, supported with direct sequencing for any mutations detected. Fragment analysis has an estimated sensitivity of approximately 2%. Any samples which have no variants detected in CALR exon 9 proceed to testing for MPL exon 10 mutations by HRM and direct sequencing.