Test Directory

Legionella Urinary Antigen Testing

Containers - Adult

20ml Sterile Universal Container
Volume Range

Ur​ine taken into Sterile Universal Container

Laboratory Site

51 Little France Crescent
Old Dalkeith Road
EH16 4SA
Telephone (Bacteriology): 0131 242 6021 OOH Bleep 2900
Howden Road West
West Lothian
EH54 6PP
Telephone (Bacteriology): 01506 52 3075 OOH contact RIE

Transport arrangements

Transport as soon as possible (within 24h)

Sample storage arrangements


If delay is anticipated, the sample should be stored between 2-8oC


Special instructions for collection

​Plain sterile univeral container.

How to request

​Use appropriate Virology Request Form (RIE) or yellow Microbiology Request Form (SJH) or via TRAK.



Weekdays 08.30-17.00

Weekends 09.00-16.00


Weekdays 09.00-17.00

Weekends 09.00-16.00

Anticipated turnaround

2 days

What happens if the result is positive or abnormal

Positive results are notified to the Duty Bacteriologist and are available in the patient’s records.

Static information/disclaimer

​Suitable for detection of Legionella pneumophila serogroup 1 antigen only

General additional information

​Clinical indication for test:

Adult patient hospitalised with severe pneumonia in NHS Lothian.

A sputum or BAL sample for the respiratory pathogen PCR panel (which includes Legionella) is the preferred sample for diagnosis as it includes several differential diagnoses. However, sputum or BAL may not always be available, and therefore urinary antigen testing may be appropriate.

Specimens will only be tested for Legionella urinary antigen if the patient meets at least one of the following criteria:
• Intensive Care patient
• Immuno-compromised/suppressed patient
• CURB65 score ≥3
• Recent foreign travel
• Spa exposure

Please contact virologyadvice@nhslothian.scot.nhs.uk for all non-urgent clinical enquiries.